Sensitivity and specificity of a rapid diagnostic test for chronic Chagas disease at a referral center in Brazil - can it be included as a standard serological diagnostic test in the clinical practice of a referral center?
DOI:
https://doi.org/10.54034/mic.e1236Keywords:
Chagas disease, rapid test, ELISA, chemoluminescence, sensitivity, specificityAbstract
Introduction: Chagas disease (CD) is a neglected tropical disease. In the chronic phase of CD, the diagnosis is essentially serologic. Conventional reactions are currently in use. More recently, the use of rapid diagnostic testing (RDT) is indicated when conventional techniques are not available. Objective: To evaluate the sensitivity and specificity of RDTs for chronic CD diagnosis. Methodology: Individuals under suspicion of CD were evaluated using ELISA, Chemiluminescence (ChLIA) and RDT tests. Results: The RDT showed 95.1% sensitivity and 96.7% specificity, respectively. Conclusion: The findings of the present study showed that RDT used in the diagnosis of CD at a referral center in Brazil were not able to detect all CD cases when compared to Elisa and ChLIA.
References
World Health Organization (WHO). Chagas disease (American trypanosomiasis) [Internet]. 2020 [cited 2020 Jul 12]. Available from: https://www.who.int/chagas/disease/en/
Martinez SJ, Romano PS, Engman DM. Precision Health for Chagas Disease: Integrating Parasite and Host Factors to Predict Outcome of Infection and Response to Therapy. Front Cell Infect Microbiol. 2020 May 8;10:210.
Dias JCP, Ramos Jr. AN, Gontijo ED, Luquetti A, Shikanai-Yasuda MA, Coura JR, et al. 2nd Brazilian Consensus on Chagas Disease, 2015. Rev Soc Bras Med Trop. 2016 Dec;49(suppl 1):3–60.
Sáez-Alquezar A, Junqueira ACV, Durans A da M, Guimarães AV, Corrêa JA, Provance Jr DW, et al. Application of WHO International Biological Reference Standards to evaluate commercial serological tests for chronic Chagas disease. Mem Inst Oswaldo Cruz. 2020;115:e200214.
Pinazo M-J, Gascon J, Alonso-Padilla J. How effective are rapid diagnostic tests for Chagas disease? Expert Review of Anti-infective Therapy. 2021 Jan 11;1–6.
Hasslocher-Moreno AM, Saraiva RM, Brasil PEAA do, Sangenis LHC, Xavier SS, Sousa AS de, et al. Temporal changes in the clinical-epidemiological profile of patients with Chagas disease at a referral center in Brazil. Rev Soc Bras Med Trop. 2021;54:e0040-2021.
Balouz V, Agüero F, Buscaglia CA. Chagas Disease Diagnostic Applications. In: Advances in Parasitology [Internet]. Elsevier; 2017 [cited 2021 Sep 18]. p. 1–45. Available from: https://linkinghub.elsevier.com/retrieve/pii/S0065308X16301038
Organización Panamericana de la Salud. Síntesis de evidencia: Guía para el diagnóstico y el tratamiento de la enfermedad de Chagas. Revista Panamericana de Salud Publica. 2020;44:e28.
Ministerio de Salud de la Nación. Guías para la atención al paciente infectado con (Trypanosoma cruzi) Enfermedad de Chagas [Internet]. Buenos Aires; 2012. Available from: https://docs.google.com/viewer?url=https%3A%2F%2Fwww.srmcursos.com%2Fpdf%2Fbiblio_medicina_GCBA2015%2FGuia_Nacional_Chagas_version_27092012.pdf
Downloads
Published
Issue
Section
License
Copyright (c) 2021 Alejandro M. Hasslocher-Moreno, Ingebourg Georg, Luiz H. C. Sangenis, Mauro F. F. Mediano
This work is licensed under a Creative Commons Attribution 4.0 International License.
How to Cite
