Rapid Identification Protocols in bacteria by MALDI-TOF from positive blood cultures. A literature review

Carolina Cucho-Espinoza; Jean Meneses-Claudio; Margaret Condori-Zevallos; Brian Meneses-Claudio

doi:10.35839/repis.7.2.1816

Autores/as

Carolina Cucho-Espinoza Facultad de Medicina Humana, Unidad de Posgrado, Universidad Nacional Mayor de San Marcos. Lima, Perú; Instituto de Investigaciones de Ciencias Biomédicas, Universidad Ricardo Palma. Lima, Perú; Servicio de Microbiología e Inmunología, Hospital Nacional Dos de Mayo. Lima, Perú https://orcid.org/0000-0003-3529-4830
Jean Meneses-Claudio Segunda especialidad en Patología Clínica, Universidad Nacional Mayor de San Marcos. Lima, Perú
Margaret Condori-Zevallos Segunda especialidad en Patología Clínica, Universidad Nacional Mayor de San Marcos. Lima, Perú https://orcid.org/0009-0005-4516-4222
Brian Meneses-Claudio Facultad de Ciencias Empresariales. Universidad Científica del Sur. Lima, Perú

DOI:

https://doi.org/10.35839/repis.7.2.1816

Palabras clave:

rapid identification, protocols, positive blood cultures, literature review, research work analysis

Resumen

Objective. To identify protocols for rapid identification of bacteria by MALDI-TOF from positive blood cultures that are as accurate as standardized ones. Methods. A bibliographic review was conducted on experimental articles, systematic reviews, experimental studies, and diagnostic test studies on the rapid identification of bacteria by MALDI-TOF from positive blood cultures. The QUADAS-2 tool was applied to evaluate the risk of bias in the articles. Results. There are various methodologies for the rapid identification of bacteria from positive blood cultures, all with a processing time of no more than one hour and with materials accessible in laboratories. These methodologies, such as Sepsityper and artisanal methods, achieve good accuracy in identifying Gram-negative bacteria, but there are still limitations in identifying Gram-positive bacteria depending on the genus and species of the bacterium. It is important to note that the quality of evidence in most studies is moderate, so the results should be taken with caution. Conclusion. There are several methodologies for rapid identification of bacteria in positive blood cultures, with short processing times and accessible materials in laboratories, that achieve good accuracy in identifying Gram-negative bacteria but have limitations in identifying Gram-positive bacteria. The quality of evidence is moderate, indicating that further studies with better design and higher quality and certainty results are needed to draw inferences obtained.

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Citas

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