Bioaugmentation Approach using Pseudomonas and Bacillus for Malodour Reduction in Poultry Feacal Waste Management

Ismail B. Onajobi; Oyindamola J. Samson; Obasola E. Fagade; Adeniyi A. Ogunjobi

doi:10.54034/mic.e1840

Authors

Ismail B. Onajobi Olabisi Onabanjo University, Ago-Iwoye, Nigeria https://orcid.org/0000-0003-1234-0259
Oyindamola J. Samson Olabisi Onabanjo University, Ago-Iwoye, Nigeria https://orcid.org/0000-0003-0404-7634
Obasola E. Fagade Department of Microbiology, Faculty of Science, University of Ibadan, Ibadan
Adeniyi A. Ogunjobi Department of Microbiology, Faculty of Science, University of Ibadan, Ibadan

DOI:

https://doi.org/10.54034/mic.e1840

Keywords:

Bioaugmentation, Seeding, Poultry, odor, Pseudomonas, Bacillus

Abstract

Introduction. A workable strategy is bioaugmentation, which involves introducing certain bacteria in sufficient quantities to promote biodegradation. This study focuses on isolating and utilizing malodor-reducing bacteria from fecal wastes obtained from a poultry farm in Ashi, Ibadan. Methods. Standard methods were employed to isolate and identify species of Pseudomonas and Bacillus. Quantitative detection of hydrogen sulfide gas and other relevant parameters was performed using MSA Orion and Multi Gas Detector. Hydrogen sulfide (H₂S) release was quantitatively monitored during fermentation, considering varying loads of inocula. Results. The bacterial isolates comprised Pseudomonas aeruginosa, P. fluorescens, P. putida, Bacillus fastidiosus, B. licheniformis, B. megaterium, B. subtilis, B. sphaericus, and B. thuringiensis. Odor levels varied based on inocula load and fermentation duration. In batches with Pseudomonas, hydrogen sulfide was undetectable after two days, while Bacillus-inoculated batches required ten days. The formation of microbial mats and subsequent decrease in H₂S content contributed to malodor reduction. Notably, fluorescent pseudomonas exhibited successful mineralization during the treatment of fecal waste. Conclusion. Pseudomonas isolates demonstrated superior effectiveness in odor reduction compared to Bacillus isolates.

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